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Dosis del etoricoxib, and placebo. They were not required to abstain from caffeine-containing beverages during the intervention phase, but caffeine consumption during placebo sessions was prohibited, and subjects completed four weeks of a moderate-intensity endurance exercise training program during the placebo days (5 d/wk, 55-60 min daily, 2 d before bed) and in-between the four weeks of endurance exercise training (2 d/wk, 75-80 min/d, 6 d/wk). The subjects were not instructed to consume caffeine-containing beverages in between the endurance exercise sessions but, to minimize the potential negative feedback effects of caffeine, the caffeine-containing beverages were served only in the morning after subjects had rested overnight and then fasted prior to exercise. A crossover design was employed, in which participants both treatment groups completed a three-period training period. Subjects underwent two baseline measurements before the endurance exercise tests (pre-exercise baseline and pre-exercise post-test), two endurance exercise tests following the three-period training period (2 d/wk, 60 min/d, 2 d in week 1 at 60 min/d), and two post-exercise measurements three weeks post-training (after six of recovery), and all were re-tested three weeks later. In addition, three-period training was combined with a one-phase supplementation period (2 d/wk, 45 min, 1 h before bed) a subsequent two-cycle aerobic training (C-T) bout, performed two days before the next two-cycle aerobic training bout; therefore, subjects in the supplement group participated a single-phase supplementation period, and subjects in the exercise group participated a two-phase training period after two post-exercise measurements three weeks apart. Measurement of Changes in Aerobic Fitness Submaximal treadmill tests (i.e. 10 km at 65% of their predicted VO 2max and submaximal graded exercise test [GTE] at 50% of their predicted VO 2max ) were performed for all subjects to assess their VO 2max, which was determined following an incremental treadmill test (T25-100, Philips Respironics, Palo ordering valacyclovir online Alto, CA) to identify their VO 2max and estimated with V̇o using a treadmill-based bio-mechanical model of body composition with the use of a gas exchange equation (24). Aerobic fitness was assessed on the treadmill using treadmill-based fitness test protocol (24). The baseline VO 2max assessment was performed 2–3 d after the end of placebo session, and the post-exercise VO 2max (EQVO ) was performed 1–3 d following the end of both treatment phases to quantify changes in aerobic fitness. VO 2max was measured using a computerized exercise test computer (model 5200) (Medtronic, Minneapolis, MN). Subjects were instructed to walk continuously up and down the treadmill (approximately 2.5 km) at a rate of 6 m·min −1 until reaching a specific test intensity for the first 3 min of test (24). In this initial phase, subjects were instructed to walk as hard they could up and down the treadmill at their predicted VO 2max during the first 3 min of treadmill cycle. The intensity valacyclovir online prices that could be achieved during the first 3 min of test was then increased in increments of 10%; then, the second, third, and fourth 3 min of exercise were incrementally increased in 10% increments, and the incremental tests were taken until subjects could no longer maintain their predicted VO 2max. Subjects were instructed to remain supine for each of the final three 3-min tests as part of a protocol intermittent-resistance treadmill walking for approximately 10 min, which was monitored by a certified fitness technician. The total distance covered during each three test minutes of exercise was recorded by a stopwatch. The exercise intensities for EQVO 2max assessment in both treatment groups were 75% of their predicted VO 2max, using the same exercise protocol; however, total miles covered in the exercise protocol was approximately 50 and 100% of predicted VO 2max, respectively. A standardized and graded treadmill was employed to quantify a series of submaximal graded exercise tests (i.e. 20 min steady-state, 30 maximum effort, 20 min recovery). For each assessment, subjects walked at their predicted Valtrex 1000 mg 60 pills $4.86 $291.87 VO 2max for each exercise at speed (i.e. a continuous treadmill at 4.5 km·h −1, 0.05 % grade ). Subjects were instructed to complete at least three graded treadmill tests for each test, with 2 d of rested condition during which time they could drink beverages; in the three-period supplementation group, subjects were instructed to not consume any caffeine or other stimulants. The first 2 d of each 2-week supplementation period consisted of a three-week aerobic exercise training session (4 d/wk, 35-45 min per day; 2 d)
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